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The Resource Biotechnology and BioChemical Engineering : Select Proceedings of ICACE 2015

Biotechnology and BioChemical Engineering : Select Proceedings of ICACE 2015

Label
Biotechnology and BioChemical Engineering : Select Proceedings of ICACE 2015
Title
Biotechnology and BioChemical Engineering
Title remainder
Select Proceedings of ICACE 2015
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Contributor
Subject
Language
eng
Cataloging source
MiAaPQ
Literary form
non fiction
Nature of contents
dictionaries
Biotechnology and BioChemical Engineering : Select Proceedings of ICACE 2015
Label
Biotechnology and BioChemical Engineering : Select Proceedings of ICACE 2015
Link
http://libproxy.rpi.edu/login?url=https://ebookcentral.proquest.com/lib/rpi/detail.action?docID=4694150
Publication
Copyright
Related Contributor
Related Location
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Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Preface -- Contents -- About the Editors -- 1 Selection of Medium and Optimization of Process Parameters for Melanin Biosynthesis from Pseudomonas stutzeri HMGM-7 -- 1 Introduction -- 2 Materials and Methods -- 2.1 Microorganisms and Culture Conditions -- 2.2 Growth Studies -- 2.3 Optimization of Nutritional Parameters for Melanin Production -- 2.4 Effect of Inoculum Age -- 2.5 Effect of Inoculum Size -- 2.6 Effect of Shaking Frequency -- 2.7 Effect of pH -- 2.8 Effect of Temperature -- 2.9 Effect of Different Growth Media -- 2.10 Effect of Carbon Sources -- 2.11 Effect of Organic Nitrogen Sources -- 2.12 Effect of l-Tyrosine -- 2.13 Extraction and Purification of Melanin -- 2.14 Characterization Studies -- 3 Results and Discussions -- 3.1 Effect of Inoculum Age -- 3.2 Effect of Inoculum Size -- 3.3 Effect of Shaking Frequency on Biomass Production -- 3.4 Effect of Shaking Frequency on Melanin Production -- 3.5 Effect of Growth Media -- 3.6 Melanin Yield Before and After Optimization -- 3.7 UV-Visible Absorption Spectra and FTIR Analysis -- 4 Conclusion -- References -- 2 Unstructured Kinetic Modeling of Glutathione Production by Saccharomyces cerevisiae NCIM 3345 -- 1 Introduction -- 2 Materials and Methods -- 2.1 Microorganism -- 2.2 Culture Maintenance -- 2.3 Fermentation -- 2.4 Biomass Determination -- 2.5 Glucose Determination -- 2.6 Glutathione (GSH) Determination -- 2.7 Fermentation Description Using Unstructured Kinetics Models -- 2.7.1 Biomass Growth -- 2.7.2 Glutathione Production Kinetics -- 2.7.3 Glucose Consumption Kinetics -- 3 Results and Discussion -- 3.1 Glutathione Production (Product Formation Kinetics) -- 3.2 Unstructured Kinetic Models -- 4 Conclusion -- Acknowledgments -- References -- 3 Statistical Optimization of Lactic Acid Extraction from Fermentation Broth Using Emulsion Liquid Membrane -- 1 Introduction
  • 2 Materials and Methods -- 2.1 Lactic Acid Production by Fermentation -- 2.1.1 Procurement of Microbial Cultures and Cell Culture Preparation -- 2.1.2 Molasses, Corn Steep Liquor and Chemicals -- 2.1.3 Fermentation Media and Immobilization of Cells -- 2.2 Emulsion Liquid Membrane -- 2.2.1 Membrane Preparation -- 2.2.2 Emulsion Stability and Lactic Acid Extraction with Molasses in External Phase -- 2.3 Lactic Acid Extraction from Fermentation Broth -- 2.4 Estimation of Total Sugar, Lactic Acid Concentration and pH -- 2.5 Calculation of Emulsion Breakage (%) -- 2.6 Calculation of Lactic Acid Extraction -- 2.7 Optimization of Lactic Acid Extraction from Fermentation Broth -- 2.7.1 Experimental Design -- 2.7.2 Experimental Procedure -- 2.7.3 Statistical Analysis and Optimization -- 3 Results and Discussion -- 3.1 Effect of Loading External Phase with Molasses on Emulsion Stability and Extraction Efficiency -- 3.2 Lactic Acid Extraction Efficiency from Fermentation Broth Using Emulsion Liquid Membrane -- 3.3 Optimization of Lactic Acid Extraction from Fermentation Broth Using Emulsion Liquid Membrane -- 3.3.1 Regression Model -- 3.4 Optimization of Lactic Acid Extraction from Fermentation Broth -- 4 Conclusion -- References -- 4 Optimization of Microwave Assisted Extraction of Pectin from Helianthus annuus Head Using Response Surface Methodology -- 1 Introduction -- 2 Materials and Methods -- 2.1 Raw Materials and Reagents -- 2.2 Extraction of Pectin Using Microwave Assisted Radiation -- 2.3 Experimental Design -- 3 Results and Discussion -- 3.1 Modeling and Optimization of Extraction Process -- 3.2 Effect of pH on Pectin Yield -- 3.3 Effect of Solid-Liquid Ratio on Pectin Yield -- 3.4 Effect of Time on Pectin Yield -- 3.5 Effect of Microwave Power on Pectin Yield -- 3.6 Determination and Validation of Optimized Conditions
  • 3.7 Characterization of Extracted Pectin by FT-IR -- 4 Conclusion -- Acknowledgments -- References -- 5 Production and Characterization of Hydrophobins from Fungal Source -- 1 Introduction -- 2 Materials and Methods -- 2.1 Microorganisms -- 2.2 Production Media -- 2.3 Quantitative Analysis -- 2.4 Molecular Weight Determination: SDS-PAGE -- 2.5 Ultrafiltration -- 3 Results and Discussion -- 3.1 Production of Hydrophobins -- 3.2 Lead Sulphide Test -- 3.3 Partial Purification by Ultrafiltration -- 4 Conclusion -- Acknowledgments -- References -- 6 Sustainable Utilization of Food Industry Waste and by-Products for the Production of Prebiotic Isomaltooligosaccharides (IMO) -- 1 Introduction -- 2 Materials and Methods -- 2.1 Chemicals and Microorganism -- 2.2 Production of Ü-Glucosidase -- 2.3 Milling of BR -- 2.4 Isolation of Starch from PPW -- 2.5 Liquefaction -- 2.6 Saccharification -- 2.7 Transglucosylation -- 2.8 Production of High Content IMO (HC-IMO) -- 2.9 HPLC Analysis -- 3 Results and Discussion -- 3.1 Liquefaction of BR and PPW -- 3.2 Saccharification of BR and PPW -- 3.3 Transglucosylation of BR and PPW -- 3.4 Production of High Content IMO -- 4 Conclusion -- Acknowledgments -- References -- 7 Enhancing the Bioproduction of Cellulase by Aspergillus nidulan via Medium Optimization -- 1 Introduction -- 2 Materials and Methods -- 2.1 Microorganisms and Maintenance -- 2.2 Inoculum Preparation -- 2.3 Fermentation Conditions -- 2.4 Enzyme Assay -- 2.5 Optimization of Cellulase Production -- 3 Results and Discussions -- 4 Conclusions -- References -- 8 Modeling and Theoretical Analysis of Isomaltooligosaccharide (IMO) Production Using Fed-Batch Process -- 1 Introduction -- 2 Methods -- 2.1 Model Development -- 2.2 Simulations -- 3 Results and Discussion -- 3.1 Constant Feeding -- 3.2 Linear Feeding -- 4 Conclusion -- Acknowledgments -- Appendix
  • References -- 9 Optimization of Na2CO3 Pre-treatment by RSM Approach for Releasing Reducing Sugars from Cocoa Pod Shells -- 1 Introduction -- 2 Methodology -- 2.1 Processing of Raw Material -- 2.2 Optimization of CPS Pre-treatment -- 2.2.1 Selection of Significant Parameters and Their Levels by OFAT -- 2.2.2 Central Composite Design -- 2.3 Validation of the Second Order Polynomial Model -- 3 Results -- 3.1 Optimisation of Pre-treatment of CPS -- 3.1.1 Selection of Significant Parameters and Their Levels by OFAT -- 3.1.2 Optimisation of Parameters for Release of Reducing Sugars by Alkali Hydrolysis by CCD -- 4 Conclusion -- Acknowledgments -- References -- 10 Partitioning of Nitralase Enzyme from Pseudomonas putida in Polymer/Salt Aqueous Two Phase System -- 1 Introduction -- 2 Materials and Methods -- 3 Results and Discussion -- 3.1 Effect of Type of Salt -- 3.2 Effect of PEG and Salt Concentration -- 3.3 Effect of Tie Line Length (TLL) -- 3.4 Effect of PH -- 3.5 Effect of Broth Loading and Phase Volume Ratio (Vr) -- 4 Conclusions -- References -- 11 Aqueous Two-Phase (Acetonitrile-Potassium Citrate) Partitioning of Bovine Serum Albumin: Equilibrium and Application Studies -- 1 Introduction -- 2 Materials and Methods -- 2.1 Materials -- 2.2 Phase Diagrams and Tie-Lines -- 2.3 Partitioning of Bovine Serum Albumin -- 3 Results and Discussion -- 3.1 Phase Diagram Data and Correlation -- 3.2 Liquid-Liquid Equilibrium Data and Correlation -- 3.3 Partitioning of Bovine Serum Albumin -- 4 Conclusions -- References -- 12 Mixed Surfactant Based Reverse Micelle Extraction of Lactose Peroxidase from Whey -- 1 Introduction -- 2 Materials and Methods -- 3 Results and Discussion -- 4 Conclusion -- References -- 13 Evaluation of Bio-surfactant on Microbial EOR Using Sand Packed Column -- 1 Introduction -- 2 Materials and Methods -- 2.1 Microorganism
  • 2.2 Media and Cultivation Conditions -- 3 Analytical Methods -- 3.1 Analysis of Sucrose Concentration -- 3.2 Cell Biomass Determination -- 3.3 Biosurfactant Determination -- 3.4 Interfacial Tension Analysis -- 3.5 Sand Packed Column Experiment -- 3.6 Satistical Analysis -- 4 Results and Discussion -- 5 Conclusions -- Acknowledgments -- References -- 14 Extraction of Polyphenols from Orange Peel by Solvent Extraction and Microbial Assisted Extraction and Comparison of Extraction Efficiency -- 1 Introduction -- 2 Materials and Methods -- 2.1 Materials -- 2.2 Samples -- 2.3 Extraction Method -- 2.4 Estimation of Total Polyphenols by Spectrophotometer Method -- 2.5 Estimation of Individual Polyphenols Using HPLC Method -- 3 Results and Discussion -- 4 Conclusion -- References -- 15 Enzymatic Concentration of n{u2212}3 Polyunsaturated Fatty Acids from Indian Sardine Oil -- 1 Introduction -- 2 Materials and Methods -- 2.1 Materials -- 2.2 Methods -- 3 Results and Discussion -- 3.1 Effect of pH on Activity of CRL -- 3.2 Effect of Temperature on the Activity of CRL -- 3.3 Effect of Oil to Water Ratio -- 3.4 Effect of Oil to Solvent Ratio -- 3.5 Effect of Time -- 3.6 Enhancement of n{u2212}3 PUFA in Oil -- 4 Conclusions -- References -- 16 High-Throughput Screening of Cell Repellent Substrate Chemistry for Application in Expanded Bed Adsorption Chromatography -- 1 Introduction -- 2 Materials and Methods -- 2.1 Chemicals and Reagents -- 2.2 Cell Culture -- 2.3 Epoxy Coating of Microwell Plate -- 2.4 Coupling of DEAE-Dextran to Epoxy Groups in Microwell Plate -- 2.5 Polymer Coating and Cell Adhesion Studies -- 3 Results and Discussion -- 3.1 PolyGMA Coating and Dextran Coupling -- 3.2 Effect of Polymer Coating on Cell Adhesion -- 4 Conclusion -- Acknowledgments -- References
  • 17 Concentration of C-Phycocyanin from Spirulina platensis Using Forward Osmosis Membrane Process
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1 online resource (233 pages)
Form of item
online
Isbn
9789811019203
Media category
computer
Media MARC source
rdamedia
Media type code
c
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remote

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