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The Resource Basic protein and peptide protocols, edited by John M. Walker

Basic protein and peptide protocols, edited by John M. Walker

Label
Basic protein and peptide protocols
Title
Basic protein and peptide protocols
Statement of responsibility
edited by John M. Walker
Contributor
Subject
Language
eng
Summary
Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the analysis of protein and peptide structure. THOUSANDS of labs are already using Basic Protein and Peptide Protocols-you should be too!
Member of
Cataloging source
YUS
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology
Series volume
v. 32
Basic protein and peptide protocols, edited by John M. Walker
Label
Basic protein and peptide protocols, edited by John M. Walker
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/089603268X
Publication
Related Contributor
Related Location
Related Agents
Related Authorities
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Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Lowry method for protein quantitation -- Bicinchoninic acid (BCA) assay for protein quantitation -- Bradford method for protein quantitation -- Nondenaturing polyacrylamide gel electrophoresis of proteins -- SDS polyacrylamide gel electrophoresis of proteins -- Gradient SDS polyacrylamide gel electrophoresis of proteins -- Acetic acid-urea polyacrylamide gel electrophoresis of proteins -- Electrophoresis of peptides -- Isoelectric focusing of proteins in ultra-thin polyacrylamide gels -- In vivo isotopic labeling of proteins for polyacrylamide gel electrophoresis -- Two-dimensional polyacrylamide gel electrophoresis of proteins -- Two-dimensional polyacrylamide gel electrophoresis using immobilized pH gradients in the first dimension -- Two-dimensional polyacrylamide gel electrophoresis using flat-bed isoelectric focusing in the first dimension -- Quantification of proteins on polyacrylamide gels (nonradioactive) -- Detection of proteins in polyacrylamide gels using an ultrasensitive silver staining technique -- identification of glycoproteins on nitrocellulose membranes and gels -- Release of oligosaccharides from glycoproteins -- Structural profiling of oligosaccharides of glycoproteins -- Dying gels -- Fluorography of polyacrylamide gels containing tritium -- Electrophoretic elution of proteins from polyacrylamide gels -- Preparation of proteins from gels for protein microsequencing -- Comparison of protein primary structures: peptide mapping -- Protein blotting -- Detection of polypeptides on immunoblots using secondary antibodies or protein A -- Detection of proteins on protein blots using chemiluminescent systems -- Detection of proteins on blots using gold or immunogold -- Amino acid analysis -- Molecular-weight estimation for native proteins using size-exclusion high-performance liquid chromatography -- Peptide purification by reverse-phase HPLC -- Enzymatic methods for cleaving proteins -- Chemical cleavage of proteins -- Chemical modification of proteins -- Dansyl method for identifying N-terminal amino acids -- Dansyl-Edman method for peptide sequencing -- C-terminal sequencing of peptides: the thiocyanate degradation method -- Analysis of cysteine residues and disulfide bonds -- Preparation and characterization of monoclonal antibodies to proteins and other cellular components -- Raising of a polyclonal antiserum to a protein -- Production of antisera to synthetic peptides -- Production of antibodies using proteins in gel bands -- Preparation of purified immunoglobulin G (IgG) -- Enzyme and biotin labeling of antibody -- Labeling of peptides and proteins by radioiodination -- Radioimmunoassay of peptides and proteins -- Enzyme-linked immunosorbent assay (ELISA) -- Free zone capillary electrophoresis
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781592595198&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3356770'}
Extent
1 online resource (xii, 490 pages)
File format
unknown
Form of item
online
Isbn
9781592595198
Lccn
93048929
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
unknown
Reproduction note
Electronic reproduction.
Sound
unknown sound
Specific material designation
remote
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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