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The Resource Basic cell culture protocols

Basic cell culture protocols

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Summary
In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of the major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis). The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today
Language
eng
Work
Publication
Edition
  • 3rd ed. /
  • edited by Cheryl D. Helgason, Cindy 1. Miller.
Extent
1 online resource (xii, 371 pages)
Contents
  • Culture of primary adherent cells and a continuously growing non-adherent cell line / Cheryl D. Helgason
  • Detection of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler
  • Eradication of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler
  • Authentication of scientific human cell lines / Wilhelm G. Dirks and Hans G. Drexler
  • Cytogenetic analysis of cell lines / Roderick A.F. MacLeod and Hans G. Drexler
  • Human and mouse hematopoeitic colony forming cell assays / Cindy L. Miller and Becky Lai
  • Isolation and culture of murine macrophages / John Q. Davies and Siamon Gordon
  • Isolation and culture of human macrophages / John Q. Davies and Siamon Gordon
  • The development of T lymphocytes in mouse fetal thymus organ culture / Tomoo Ueno [and others]
  • In vitro generation of lymphocytes from embryonic stem cells / Renée F. de Pooter, Sarah K. Cho and Juan Carlos Zúniga-Pflücker
  • Hematopoietic development of human embryonic stem cells in culture / Xinghui Tian and Dan S. Kaufman
  • Generation of murine stromal cell lines / Robert A.J. Oostendorp, Kirsty Harvey and Elaine E. Dzierzak
  • Culture of human and mouse mesenchymal cells / Emer Clarke
  • Isolation, purification, and cultivation of rat and human keratinocytes / Frizell L. Vaughan and Ludmila I. Bernstam
  • Isolation and culture of primary human hepatocytes / Edward L. LeCluyse [and others]
  • Primary kidney proximal tubule cells / Mary Taub
  • Enzymatic dissociation and culture of normal human mammary tissue to detect progenitor activity / John Stingl, Joanne T. Emerman, and Connie J. Eaves
  • Generation and differentiation of neurospheres from murine embryonic day 14 central nervous system tissue / Sharon A. Louis and Brent A. Reynolds
  • Isolation and culture of skeletal muscle myofibers as a means to analyze satellite cells / Gabi Shefer and Zipora Yablonka-Reuveni
  • Adult ventricular cardiomyocytes / Klaus-Dieter Schlüter and Daniela Schreiber
  • Isolation and culture of primary endothelial cells / Bruno Larrivée and Aly Karsan
  • Studying leukocyte rolling and adhesion in vitro under flow conditions / Susan L. Cuvelier and Kamala D. Patel
  • Isolation and characterization of side population cells / Margaret A. Goodell, Shannon McKinney-Freeman and Fernando D. Camargo
  • Scalable production of embryonic stem cell-derived cells / Stephen M. Dang and Peter W. Zandstra
Isbn
9781423714859
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/1592598382
Instance
Label
Basic cell culture protocols
Title
Basic cell culture protocols
Contributor
Subject
Genre
Language
eng
Summary
In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of the major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis). The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today
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Cataloging source
COO
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology
Series volume
290
Basic cell culture protocols
Label
Basic cell culture protocols
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/1592598382
Publication
Related Contributor
Related Location
Related Agents
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Culture of primary adherent cells and a continuously growing non-adherent cell line / Cheryl D. Helgason -- Detection of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler -- Eradication of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler -- Authentication of scientific human cell lines / Wilhelm G. Dirks and Hans G. Drexler -- Cytogenetic analysis of cell lines / Roderick A.F. MacLeod and Hans G. Drexler -- Human and mouse hematopoeitic colony forming cell assays / Cindy L. Miller and Becky Lai -- Isolation and culture of murine macrophages / John Q. Davies and Siamon Gordon -- Isolation and culture of human macrophages / John Q. Davies and Siamon Gordon -- The development of T lymphocytes in mouse fetal thymus organ culture / Tomoo Ueno [and others] -- In vitro generation of lymphocytes from embryonic stem cells / Renée F. de Pooter, Sarah K. Cho and Juan Carlos Zúniga-Pflücker -- Hematopoietic development of human embryonic stem cells in culture / Xinghui Tian and Dan S. Kaufman -- Generation of murine stromal cell lines / Robert A.J. Oostendorp, Kirsty Harvey and Elaine E. Dzierzak -- Culture of human and mouse mesenchymal cells / Emer Clarke -- Isolation, purification, and cultivation of rat and human keratinocytes / Frizell L. Vaughan and Ludmila I. Bernstam -- Isolation and culture of primary human hepatocytes / Edward L. LeCluyse [and others] -- Primary kidney proximal tubule cells / Mary Taub -- Enzymatic dissociation and culture of normal human mammary tissue to detect progenitor activity / John Stingl, Joanne T. Emerman, and Connie J. Eaves -- Generation and differentiation of neurospheres from murine embryonic day 14 central nervous system tissue / Sharon A. Louis and Brent A. Reynolds -- Isolation and culture of skeletal muscle myofibers as a means to analyze satellite cells / Gabi Shefer and Zipora Yablonka-Reuveni -- Adult ventricular cardiomyocytes / Klaus-Dieter Schlüter and Daniela Schreiber -- Isolation and culture of primary endothelial cells / Bruno Larrivée and Aly Karsan -- Studying leukocyte rolling and adhesion in vitro under flow conditions / Susan L. Cuvelier and Kamala D. Patel -- Isolation and characterization of side population cells / Margaret A. Goodell, Shannon McKinney-Freeman and Fernando D. Camargo -- Scalable production of embryonic stem cell-derived cells / Stephen M. Dang and Peter W. Zandstra
http://library.link/vocab/cover_art
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http://library.link/vocab/discovery_link
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Edition
  • 3rd ed. /
  • edited by Cheryl D. Helgason, Cindy 1. Miller.
Extent
1 online resource (xii, 371 pages)
Form of item
online
Isbn
9781423714859
Lccn
2004011235
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Specific material designation
remote

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