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The Resource Astrocytes : methods and protocols, edited by Richard Milner

Astrocytes : methods and protocols, edited by Richard Milner

Astrocytes : methods and protocols
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methods and protocols
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edited by Richard Milner
Ever since their discovery more than 150 years ago, astrocytes have proved to be something of an enigma. Only in the last 10-15 years has it become clear that astrocytes are multifunctional versatile cells that play key roles in a multitude of diverse processes in the CNS. Astrocytes: Methods and Protocols provides scientists with a comprehensive guide to many techniques used for astrocyte cell culture, as well as more specialized approaches for studying astrocyte functions, both in vitro and in vivo. Key cellular, molecular and biochemical techniques are used to study the many and varied functions of this fascinating cell. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical Astrocytes: Methods and Protocols seeks to aid scientists and researchers looking to use more specialized techniques to examine astrocyte function
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non fiction
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  • dictionaries
  • bibliography
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Methods in molecular biology,
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Astrocytes : methods and protocols, edited by Richard Milner
Astrocytes : methods and protocols, edited by Richard Milner
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Includes bibliographical references and index
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online resource
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Astrocytes : multitalented stars of the central nervous system / Bruce R. Ransom and Christoher B. Ransom -- Neurogenic astrocytes and their glycoconjugates : not just "glue" anymore / Dennis A. Steindler -- Heterogeneity of astrocytic form and function / Nancy Ann Oberheim, Steven A. Goldman, and Maiken Nedergaard -- Preparation of mixed glial cultures from postnatal rat brain / Jean de Vellis and Ruth Cole -- Generation of primary astrocyte cultures devoid of contaminating microglia / Tracy F. Uliasz [and others] -- Derivation of microglia-free astrocyte cultures from neural stem cells / Jennifer V. Welser and Richard Milner -- Isolation and culture of spinal cord astrocytes / Amber E. Kerstetter and Robert H. Miller -- Isolation and culture of astrocytes from the retina and optic nerve / Thomas J. Lukas and Ai Ling Wang -- Primary cultures of astrocytes from fetal bovine brain / Cristina Ballarin and Antonella Peruffo -- Isolation and cultivation of porcine astrocytes / Dennis J. Bobilya -- Isolation and culture of human astrocytes / Ariane Sharif and Vincent Prevot -- Cell culturing of Caenorhabditis elegans glial cells for the assessment of cytosolic Ca2 dynamics / Randy F. Stout Jr. and Vladimir Parpura -- Astrocyte polarization and wound healing in culture : studying cell adhesion molecules / H. Peng and S. Caronetto -- An in vitro trauma model to study rodent and human astrocyte reactivity / Ina-Beate Wanner -- Use of gel zymography to examine matrix metalloproteinase (gelatinase) expression in brain tissue or in primary glial cultures / Harald Frankowski [and others] -- ELISA methodology to quantify astrocyte production of cytokines/chemokines in vitro / Hongwei Qin and Etty N. Benveniste -- Induction of nitric oxide synthase-2 expression and measurement of nitric oxide production in enriched primary cortical astrocyte cultures / James A. Hewett and Sandra J. Hewett -- Examining potassium channel function in astrocytes / Michelle Olsen -- Connexin-based channels in astrocytes : how to study their properties / Christian Giaume [and others] -- Isotope-based quantitation of uptake, release, and metabolism of glutamate and glucose in cultured astrocytes / Leif Hertz -- An in vitro model of reactive astrogliosis and its effect on neuronal growth / Panpan Yu [and others] -- A neuron-astrocyte co-culture system to investigate astrocyte-secreted factors in mouse neuronal development / Emma V. Jones, Denise Cook, and Keith K. Murai -- Cocultures of neurons and astrocytes as a model for examining hypoxia-induced neuronal death / Giuliano Rita, Terho Emily, and David A. Rempe -- Use of astrocyte-microglial cocultures to examine the regulatory influence of astrocytes on microglial activation / Jennifer V. Welser and Richard Milner -- Astrocyte-Schwann-cell coculture systems / Fardad T. Afshari and James W. Fawcett -- An in vitro assay to examine oligodendrocyte precursor cell migration on astrocytes / Fardad T. Afshari and James W. Fawcett -- Investigation of astrocyte-oligodendrocyte interactions in human cultures / Gareth R. John -- An improved in vitro blood-brain barrier model : rat brain endothelial cells co-cultured with astrocytes / N. Joan Abbott [and others] -- In vitro models of the blood-brain barrier / Kaushik K. Shah, Li Yang, and Thomas J. Abbruscato -- An in vitro model of ischemic stroke / Li Yang, Kaushik K. Shah, and Thomas J. Abbruscato -- Pericyte coculture models to study astrocyte, pericyte, and endothelial cell interactions / Vladimir Katyshev and Paula Dore-Duffy -- Reprogramming of postnatal astroglia of the mouse neocortex into functional, synapse-forming neurons / Christophe Heinrich, Magdalena Götz, and Benedikt Berninger -- Assessment of glial function in the in vivo retina / Anja I. Srienc [and others] -- Imaging in vivo astrocyte/endothelial cell interactions at the blood-brain barrier / Colin L. Willis -- Transgenic techniques for cell ablation or molecular deletion to investigate functions of astrocytes and other GFAP-expressing cell types / Michael V. Sofroniew -- In vivo imaging of Ca2 signaling in astrocytes using two-photon laser scanning fluorescent microscopy / Shinghua Ding -- Use of Cre-lox technology to analyze integrin functions in astrocytes / Aaron K. Mobley and Joseph H. McCarty
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1 online resource (xiv, 575 pages)
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