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The Resource Antibody phage display : methods and protocols, edited by Philippa M. O'Brien and Robert Aitken

Antibody phage display : methods and protocols, edited by Philippa M. O'Brien and Robert Aitken

Label
Antibody phage display : methods and protocols
Title
Antibody phage display
Title remainder
methods and protocols
Statement of responsibility
edited by Philippa M. O'Brien and Robert Aitken
Contributor
Subject
Genre
Language
eng
Summary
Antibody phage display, the definitive technology for monoclonal production, has now advanced to a stage where it can be performed in nonspecialized research laboratories. In Antibody Phage Display: Methods and Protocols, Philippa M. O'Brien and Robert Aitken combine in one volume a comprehensive collection of established antibody phage display protocols, each accompanied by authoritative guidance that will enable the nonspecialist to carry them out successfully. Coverage spans the construction of antibody libraries, the selection of antibody clones with the desired properties, and their modification, expression, and purification. Each readily reproducible method is described by a hands-on expert in step-by-step detail and includes a wealth of practical advice not found in the scientific literature. Extensive notes discuss pitfalls to avoid and offer many possible alternative methods to suit special research situations. An overview by one of the world's leading experts in antibody phage display, Dr. Hennie R. Hoogenboom, surveys the current status of the field and the future of the technology. Comprehensive and highly practical, Antibody Phage Display: Methods and Protocols provides biochemists, molecular biologists, and immunologists with a gold-standard reference guide to the successful isolation, modification, and expression of recombinant antibodies using today's powerful phage display technology
Member of
Cataloging source
COO
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology
Series volume
v. 178
Antibody phage display : methods and protocols, edited by Philippa M. O'Brien and Robert Aitken
Label
Antibody phage display : methods and protocols, edited by Philippa M. O'Brien and Robert Aitken
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/1592592406
Publication
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Innovative proteomics for the discovery of systemically accessible cancer biomarkers suitable for imaging and targeted therapies -- A capillary electrophoresis method for evaluation of abeta proteolysis in vitro -- Exercise-induced HSP27, HSP70 and mapk responses in human skeletal muscle -- Transcriptome analysis of the response to chronic constant hypoxia in zebrafish hearts -- Tuberculosis outbreaks predicted by characteristics of first patients in a DNA fingerprint cluster -- Aromatase inhibitors: Past, present and future -- The recovery of immunoglobulin sequences from single human B cells by clonal expansion -- Strength, workload, anaerobic intensity and the immune response to resistance exercise in women -- Uptake and intracellular distribution of various metal ions in human monocyte-derived dendritic cells detected by newport green DCF diacetate ester -- A rapid method purifies a glycoprotein of MR 145,000 as the LDL receptor of trypanosoma brucei brucei -- Diagnostic value of different PCR assays for the detection of mycobacterial DNA in granulomatous lymphadenopathy -- Glutaraldehyde fixation increases retention of low molecular weight proteins (growth factors) transferred to nylon membranes for western blot analysis -- [molecular imaging of apoptosis and necrosis -- basic principles of cell biology and use in oncology] -- Allelic exchange in mycobacterium tuberculosis with long linear recombination substrates -- Use of a volatile buffer system in ion-exchange high-performance liquid chromatography of oligonucleotides -- Gender-specific effects of estrogen receptor alpha gene haplotype on high-density lipoprotein cholesterol response to atorvastatin: Interaction with apolipoprotein ai gene polymorphism -- Single cell assay with an automated capillary microinjection system -- Cyclin E overexpression impairs progression through mitosis by inhibiting APC(cdh1) -- Peptide mapping by CNBR fragmentation using a sodium dodecyl sulfate-polyacrylamide minigel system -- The "new genetics" and clinical practice -- NMR studies of the conformations of leghemoglobins from soybean and lupin -- Thin-layer chromatographic method for the determination of glycosyltransferase activity -- Formation of transient polykaryons by fusion of erythrocytes of different developmental programs -- Sputum proteomics in inflammatory and suppurative respiratory diseases -- Visualization of reversible macromolecular reactions in an analytical ultracentrifuge -- Bearing of genetic data on the interpretation of the subunit structure of octanol dehydrogenase of drosophila -- Phosphoproteomic analysis of signaling pathways in head and neck squamous cell carcinoma patient samples -- Influences of hydraulic loading rate on svoc removal and microbial community structure in drinking water treatment biofilters -- Detection of leishmania DNA by polymerase chain reaction in scars of treated human patients -- Identification of 3',5'-cyclic amp in axenic cultures of lemna paucicostata by high-performance liquid chromatography -- Regulatory t cell vaccination without autoantigen protects against experimental autoimmune encephalomyelitis -- In situ visualization of high genetic diversity in a natural microbial community -- Use of PCR to isolate genes encoding sigma54-dependent activators from diverse bacteria -- Insertional mutagenesis and recovery of interrupted genes of streptococcus mutans by using transposon TN917: Preliminary characterization of mutants displaying acid sensitivity and nutritional requirements -- Periodic phenomena in proteus mirabilis swarm colony development -- Genomics and diversity of the common marmoset monkey NK complex
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Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3356710'}
Extent
1 online resource (xiii, 401 pages)
Form of item
online
Isbn
9780896039063
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Specific material designation
remote

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