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The Resource Antibody engineering protocols, edited by Sudhir Paul

Antibody engineering protocols, edited by Sudhir Paul

Label
Antibody engineering protocols
Title
Antibody engineering protocols
Statement of responsibility
edited by Sudhir Paul
Contributor
Subject
Language
eng
Summary
This comprehensive collection of recently developed methods for producing new antibody reagents by immunization and recombinant DNA techniques contains ready-to-use protocols that illuminate current areas of research on antibody structure, functions, and applications. The methods can be applied in basic immunological studies involving antibody specificity, catalysis, and evolution, and in the isolation of rare antibodies by phage display technology and the engineering of new antibodies by mutagenesis. They offer insight into new ways of developing clinically useful antibody reagents. Antibody Engineering Protocols constitutes a single-source volume for laboratory investigators who want to minimize extensive literature and methodology searches and to work productively in their fields with reproducible step-by-step protocols
Member of
Cataloging source
YNG
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology
Series volume
v. 51
Target audience
adult
Antibody engineering protocols, edited by Sudhir Paul
Label
Antibody engineering protocols, edited by Sudhir Paul
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/0896032752
Publication
Related Contributor
Related Location
Related Agents
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Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
SEQHUNT : a program to screen aligned nucleotide and amino acid sequences / George Johnson, Tai Te Wu, Elvin A. Kabat -- Molecular modeling of antibody-combining sites / David M. Webster, Anthony R. Rees -- Structure and properties of human immunoglobulin light-chain dimers / Fred J. Stevens, Marianne Schiffer -- Crystallographic and chromatographic methods for study of antibody light chains and other proteins / Marianne Schiffer, Fred J. Stevens -- Detection of human variable gene family expression at the single-cell level / Moncef Zouali -- Purification of reduced and alkylated antibody subunits / Mei Sun, Sudhir Paul -- Murine monoclonal antibody development / Donald R. Johnson -- Comparative properties of polyclonal and monoclonal antibodies / L. Scott Rodkey -- Evaluation of antibody clonality / L. Scott Rodkey -- Affinity immunoblotting / Keith A. Knisley, L. Scott Rodkey -- Epitope and idiotope mapping using monoclonal antibodies / Srinivas Kaveri -- Anti-idiotypic antibodies that mimic opioids / Jay A. Glasel, Dianne Agarwal -- Catalytic antibodies : structure and possible applications / Howard Amital, Ilan Tur-Kaspa, Zeev Tashma, Israel Hendler, Yehuda Shoenfeld -- Preparation and assay of acetylcholinesterase antibody / Alain Friboulet, Ladan Izadyar-Demichèle -- DNA hydrolysis by antibodies / Alexander G. Gabibov, Oxana Makarevitch -- Screening strategies for catalytic antitransition-state analog antibodies / Alfonso Tramontano -- Expression of chimeric immunoglobulin genes in mammalian cells / Sergey M. Deyev, Oleg L. Polanovsky -- Single-chain anti-DNA Fv / Michael Polymenis, B. David Stollar -- Molecular cloning of antiground-state proteolytic antibody fragments / Qing-Sheng Gao, Sudhir Paul -- Cloning and bacterial expression of an esterolytic sFv / Rodger G. Smith, Mark T. Martin, Rosa Sanchez, John H. Kenten -- Site-directed mutagenesis of antibody-variable regions / Qing-Sheng Gao, Sudhir Paul -- Synthetic antibody gene libraries for in vitro affinity maturation / Su-jun Deng, C. Roger MacKenzie, Saran A. Narang -- Chaperonins in phage display of antibody fragments / Eskil Söderlind, Marta Dueñas, Carl A. Borrebaeck -- Phage-display libraries of murine and human antibody fab fragments / Jan Engberg, Peter Sejer Andersen, Leif Kofoed Nielsen, Morten Dziegiel, Lene K. Johansen, Bjarne Albrechtsen -- Selection of human immunoglobulin light chains from a phage-display library / Sonia Tyutyulkova, Qing-Sheng Gao, Sudhir Paul -- Purification of antibody light chains by metal affinity and protein L chromatography / Sonia Tyutyulkova, Sudhir Paul -- Rapid purification of recombinant antibody fragments for catalysis screening / Han Huang, Brian Fichter, Robert Dannenbring, Sudhir Paul -- Assay of radiolabeled VIP binding and hydrolysis by antibodies / Han Huang, Sudhir Paul -- Methods of measuring thyroglobulin and peptide-methylcoumarinamide hydrolysis by autoantibodies / Lan Li, Ravishankar Kalaga, Srinivas Kaveri, Sudhir Paul -- Radiolabeling of antibodies for therapy and diagnosis / Janina Baranowska-Kortylewicz, Glenn V. Dalrymple, Syed M. Quadri, Katherine A. Harrison
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781592595389&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357153'}
Extent
1 online resource (xii, 450 pages)
File format
one file format
Form of item
online
Isbn
9781592595389
Lccn
95000423
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
access
Specific material designation
remote

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