Coverart for item
The Resource Animal cell electroporation and electrofusion protocols, edited by Jac A. Nickoloff

Animal cell electroporation and electrofusion protocols, edited by Jac A. Nickoloff

Label
Animal cell electroporation and electrofusion protocols
Title
Animal cell electroporation and electrofusion protocols
Statement of responsibility
edited by Jac A. Nickoloff
Title variation
Animal cell electroporation & electrofusion protocols
Contributor
Subject
Genre
Language
eng
Summary
This superb collection of well-tested protocols for the electroporation of proteins and DNA into insect, fish, and mammalian cells also includes proven animal cell electrofusion techniques for studies of somatic cell genetics and development, and for generating monoclonal antibodies. It is distinguished by its coverage of important model cell types from many organisms and tissue types and its detailed instructions for the growth and preparation of specific cells to achieve optimum transfection. Its extensive reference lists, citations of alternative transfer methods, advice on pitfalls to avoid, and descriptions of expected results ensure readily reproducible results even for beginners
Member of
Cataloging source
YNG
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology
Series volume
v. 48
Target audience
adult
Animal cell electroporation and electrofusion protocols, edited by Jac A. Nickoloff
Label
Animal cell electroporation and electrofusion protocols, edited by Jac A. Nickoloff
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/089603304X
Publication
Related Contributor
Related Location
Related Agents
Related Authorities
Related Subjects
Related Items
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Electroporation theory : concepts and mechanisms / James C. Weaver -- Effects of pulse length and strength on electroporation efficiency / Sek Wen Hui -- Instrumentation / Gunter A. Hofmann -- The introduction of proteins into mammalian cells by electroporation / William F. Morgan, Joseph P. Day -- Electroporation of antigen-presenting cells for T-cell recognition and cytotoxic T-lymphocyte priming / Weisan Chen, James McCluskey -- Electroporation of antibodies into mammalian cells / Paul L. Campbell, James McCluskey, Jing Ping Yeo, Ban-Hock Toh -- Electroporation of adherent cells in situ for the introduction of nonpermeant molecules / Leda H. Raptis, Kevin L. Firth, Heather L. Brownell, Andrea Todd, W. Craig Simon, Brian M. Bennett, Leslie W. MacKenzie, Maria Zannis-Hadjopoulos -- Electrotransformation of Chinese hamster ovary cells / Danielle Gioioso Taghian, Jac A. Nickoloff -- Electroporation of rat pituitary cells / Ruth H. Paulssen, Eyvind J. Paulssen, Kaare M. Gautvik -- Electroporation of plasmid DNA into normal human fibroblasts / F. Andrew Ray -- Electroporation-mediated gene transfer into hepatocytes / Alphonse Cam -- Electroporation of human lymphoblastoid cells / Fen Xia, Howard L. Liber -- The use of electroporated bovine spermatozoa to transfer foreign DNA into oocytes / Marc Gagné, François Pothier, Marc-André Sirard -- Electroporation of embryonic stem cells for generating transgenic mice and studying in vitro differentiation / John S. Mudgett, Thomas J. Livelli -- Electrotransfection with "intracellular" buffer / Maurice J. Hoff, Vincent M. Christoffels, Wil T. Labruyère, Antoon F. Moorman, Wouter H. Lamers -- Effect of cis-located human satellite DNA on electroporation efficiency / Djenann Saint-Dic, Michael S. DuBow -- Quantitation of transient gene expression / Michael K. Showe, Louise C. Showe -- Stable integration of vectors at high copy number for high-level expression in animal cells / James Barsoum -- Electroporation of Drosophila embryos / K. Puloma Kamdar, Thao N. Wagner, Victoria Finnerty -- Transformation of fish cells and embryos / Koji Inoue, Jun-ichiro Hata, Shinya Yamashita -- Electroporation of cardiac cells / Leslie Tung -- Electroporation for gene therapy / Kathryn E. Matthews, Sukhendu B. Dev, Frances Toneguzzo, Armand Keating -- Electrofusion of mammalian cells / Kenneth L. White -- Stabilizing antibody secretion of human Epstein Barr virus-activated B-lymphocytes with hybridoma formation by electrofusion / Susan Perkins, Steven K. Foung -- Electrofusion of mammalian oocytes and embryonic cells / Josef Fulka, Robert M. Moor, Josef Fulka -- Nuclear transfer in bovine embryos / Akira Iritani, Tasuku Mitani -- Electrofusion of mouse embryos to produce tetraploids / Ulrich Petzoldt -- Spectrofluorometric assay for cell-tissue electrofusion / Richard Heller -- Cytometric detection and quantitation of cell-cell electrofusion products / Mark J. Jaroszeski, Richard Gilbert, Richard Heller
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781592595358&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357149'}
Extent
1 online resource (xx, 369 pages)
File format
one file format
Form of item
online
Isbn
9781592595358
Lccn
95030825
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
access
Specific material designation
remote

Library Locations

    • Folsom LibraryBorrow it
      110 8th St, Troy, NY, 12180, US
      42.729766 -73.682577
Processing Feedback ...