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The Resource Advanced technologies for protein complex production and characterization, M. Cristina Vega, editor

Advanced technologies for protein complex production and characterization, M. Cristina Vega, editor

Advanced technologies for protein complex production and characterization
Advanced technologies for protein complex production and characterization
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M. Cristina Vega, editor
This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression of E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists. Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methyltrophic and non-methyltrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammaliancells, plants and algae. Complex reconstruction from individually purified subunits or subcomplexes is discussed as a complimentary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance. This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression system to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies -- Back of book
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A39 2016
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non fiction
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Advances in experimental medicine and biology,
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volume 896
Advanced technologies for protein complex production and characterization, M. Cristina Vega, editor
Advanced technologies for protein complex production and characterization, M. Cristina Vega, editor
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Includes bibliographical references and index
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Part I: Introduction -- Protein complex production from the drug discovery standpoint / Ismail Moarefi -- Choose a suitable expression host: a survey of available protein production platforms / Francisco J. Fernández and M. Cristina Vega -- Part II: Prokaryotic expression hosts -- ACEMBL tool-kits for high-throughput multigene delivery and expression in prokaryotic and eukaryotic hosts / Yan Nie, Maxime Chaillet, Christian Becke, Matthias Haffke, Martin Pelosse, Daniel Fitzgerald, Ian Collinson, Christiane Schaffitzel, and Imre Berger -- Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput / Renaud Vincentelli and Christophe Romier -- Membrane protein production in E. coli for applications in drug discovery / Harm Jan (Arjan) Snijder and Jonna Hakulinen -- Cell-free synthesis of macromolecular complexes / Mathieu Botte, Aurélien Deniaud, and Christiane Schaffitzel -- A Bacillus magaterium system for the production of recombinant proteins and protein complexes / Rebekka Biedendieck -- Protein complex production in alternative prokaryotic hosts / Sara Gómez, Miguel López-Estepa, Francisco J. Fernández, and M. Cristina Vega -- Part III: Lower eukaryotic expression hosts -- Production of protein complexes in non-methylotrophic and methylotrophic yeasts / Francisco J. Fernández, Miguel López-Estepa, Javier Querol-García, and M. Cristina Vega -- Leishmania tarentolae for the production of multi-subunit complexes / Tomoaki Niimi -- Alternative eukaryotic expression systems for the production of proteins and protein complexes / Sara Gómez, Miguel López-Estepa, Francisco J. Fernández, Teresa Suárez, and M. Cristina Vega -- Part IV: Higher eukaryotic expression hosts -- Fundamentals of baculovirus expression and applications / Thomas A. Kost and Christopher W. Kemp -- The MultiBac baculovirus/insect cell expression vector system for producing complex protein biologics / Duygu Sari, Gapil Gupta, Deepak Balaji Thimiri Govinda Raj, Alice Aubert, Petra Drncová, Frederic Garzoni, Daniel Fitzgerald, and Imre Berger -- Fundamentals of expression in mammalian cells / Michael R. Dyson -- Assembling multi-subunit complexes using mammalian expression / Bahar Baser and Joop van den Heuvel -- Part V: Plant expression -- Microalgae as solar-powered protein factories / Franziska Hempel and Uwe G. Maier -- Strategies and methodologies for the co-expression of multiple proteins in plants / Albert Ferrer, Monserrat Arró, David Manzano, and Teresa Altabella -- Transient expression systems in plants: potentialities and constraints / Tomas Canto -- Part VI: Complex reconstitution -- Complex reconstitution from individual protein modules / Jérôme Basquin, Michael Taschner, and Esben Lorentzen -- Structural reconstitution of protein-protein complexes involved in intracellular signaling / Klára Kirsch, Péter Sok, and Attila Reményi -- Part VII: Biophysical methods to assess protein complexes -- The use of small-angle scattering for the characterization of multi subunit complexes / Adam Round -- Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems / Filippo Prischi and Annalisa Pastore
27 cm.
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xiii, 382 pages
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